PittsburghV1, Main, Exploration, bibRecord, 003824

Combining Perfluorocarbon and Superparamagnetic Iron-oxide Cell Labeling for Improved and Expanded Applications of Cellular MRI

Identifieur interne : 003824 ( Main/Exploration ); précédent : 003823; suivant : 003825

Combining Perfluorocarbon and Superparamagnetic Iron-oxide Cell Labeling for Improved and Expanded Applications of Cellular MRI

Auteurs : T. Kevin Hitchens [États-Unis] ; Li Liu [États-Unis] ; Lesley M. Foley [États-Unis] ; Virgil Simplaceanu [États-Unis] ; Eric T. Ahrens [États-Unis] ; Chien Ho [États-Unis]

Source :

Magnetic resonance in medicine [ 0740-3194 ] ; 2014.

RBID : PMC:4115051

Descripteurs français

English descriptors

KwdEn :
- Animals, Cell Tracking (methods), Contrast Media, Dextrans, Feasibility Studies, Fluorocarbons, Image Enhancement (methods), Inflammation (pathology), Macrophages (pathology), Magnetic Resonance Imaging (methods), Magnetite Nanoparticles, Male, Mice, Mice, Inbred C57BL, Reproducibility of Results, Sensitivity and Specificity, Staining and Labeling (methods).
MESH :
- chemical : Contrast Media, Dextrans, Fluorocarbons, Magnetite Nanoparticles.
- methods : Cell Tracking, Image Enhancement, Magnetic Resonance Imaging, Staining and Labeling.
- pathology : Inflammation, Macrophages.
- Animals, Feasibility Studies, Male, Mice, Mice, Inbred C57BL, Reproducibility of Results, Sensitivity and Specificity.

Abstract

Purpose

The ability to detect the migration of cells in living organisms is fundamental in understanding biological processes and important for the development of novel cell-based therapies to treat disease. MRI can be used to detect the migration of cells labeled with superparamagnetic iron-oxide (SPIO) or perfluorocarbon (PFC) agents. In this study, we explored combining these two cell-labeling approaches to overcome current limitations and enable new applications for cellular MRI.

Methods

We characterized ¹⁹F-NMR relaxation properties of PFC-labeled cells in the presence of SPIO and imaged cells both ex vivo and in vivo in a rodent inflammation model to demonstrate selective visualization of cell populations.

Results

We show that with UTE3D, RARE and FLASH ¹⁹F images one can uniquely identify PFC-labeled cells, co-localized PFC- and SPIO-labeled cells, and PFC/SPIO co-labeled cells.

Conclusion

This new methodology has the ability to improve and expand applications of MRI cell tracking. Combining PFC and SPIO strategies can potentially provide a method to quench PFC signal transferred from dead cells to macrophages, thereby eliminating false positives. In addition, combining these techniques could also be used to track two cell types simultaneously and probe cell-cell proximity in vivo with MRI.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4115051

DOI: 10.1002/mrm.25120
PubMed: 24478194
PubMed Central: 4115051

Affiliations:

Le document en format XML

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<front><div type="abstract" xml:lang="en"><sec id="S1"><title>Purpose</title>
<p id="P2">The ability to detect the migration of cells in living organisms is fundamental in understanding biological processes and important for the development of novel cell-based therapies to treat disease. MRI can be used to detect the migration of cells labeled with superparamagnetic iron-oxide (SPIO) or perfluorocarbon (PFC) agents. In this study, we explored combining these two cell-labeling approaches to overcome current limitations and enable new applications for cellular MRI.</p>
</sec>
<sec id="S2"><title>Methods</title>
<p id="P3">We characterized <sup>19</sup>
F-NMR relaxation properties of PFC-labeled cells in the presence of SPIO and imaged cells both <italic>ex vivo</italic>
 and <italic>in vivo</italic>
 in a rodent inflammation model to demonstrate selective visualization of cell populations.</p>
</sec>
<sec id="S3"><title>Results</title>
<p id="P4">We show that with UTE3D, RARE and FLASH <sup>19</sup>
F images one can uniquely identify PFC-labeled cells, co-localized PFC- and SPIO-labeled cells, and PFC/SPIO co-labeled cells.</p>
</sec>
<sec id="S4"><title>Conclusion</title>
<p id="P5">This new methodology has the ability to improve and expand applications of MRI cell tracking. Combining PFC and SPIO strategies can potentially provide a method to quench PFC signal transferred from dead cells to macrophages, thereby eliminating false positives. In addition, combining these techniques could also be used to track two cell types simultaneously and probe cell-cell proximity <italic>in vivo</italic>
 with MRI.</p>
</sec>
</div>
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Combining Perfluorocarbon and Superparamagnetic Iron-oxide Cell Labeling for Improved and Expanded Applications of Cellular MRI

Combining Perfluorocarbon and Superparamagnetic Iron-oxide Cell Labeling for Improved and Expanded Applications of Cellular MRI

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Descripteurs français

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Abstract

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